In vitro selection of NaCl tolerant lines of Lycopersicon esculentum Mill.
1985
Thawanrat Phongsai
Leaf explants of two tomato cultivars, VF 134-1-2 and P 5-237, were cultured in vitro on a Murashige and Skoogs (1962) (MS) medium supplemented with 1 mg/1 IAA (alpha-indoleacetic acid), 2.5 mg/1 BA (6-benzyladenine) and sodium chloride (NaCl) at 0, 1, 1.5, 2, 2.5 or 3 percent. Eighty-six and twenty percent of the leaf explants of VF 134-1-2 grew and formed callus on the medium containing 0 and 1 percent NaCl, respectively. Leaf explants of P 5-237 cultivar failed to form callus on the NaCl-medium. In another experiment, leaf explants were induced to form callus on a MS medium supplemented with 1 mg/1 IAA and 2.5 mg/1 BA for 2 weeks. Then, the callus tissue were transferred onto the same medium formula but with the addition of NaCl at 0, 1, 1.5, 2, 2.5 or 3 percent. Callus growth, as measured by fresh weight after 4 weeks, increased inversely proportional to NaCl concentrations. Nevertheless, on the medium containing 1 percent NaCl, shoots, if any, were formed on the explants of VF 134-1-2 and P 5-237 cultivars, but shoots growth were inhibited. When they were transferred onto the medium having no NaCl, shoot growth occurred normally. Roots were induced to form on a basal MS medium and the plantlets were successfully transplanted to soil
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