A genetic map of the R-factor pKU10 isolated from Pseudomonas putida
1988
Yim, Y.B. | Min, K.M. | Lee, Y.N. (Korea Univ., Seoul (Korea R.). Dept. of Biology)
A genetic map of the IncP-1 group plasmid pKU10 has been prepared through the construction of recombinant plasmids containing various fragments of pKU10. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to ampicillin, tetracycline, and chloramphenicol. The region involved in conferring resistance to ampicillin was located around two PstI sites that are 1.0Kb apart. The tetracycline resistance gene was mapped on the region of HindIII E fragment and a part of HindIII D fragment, and the determinant for chloramphenicol resistance gene was localized on HindIII D fragment
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