Characterization of R plasmid pKU41 from Pseudomonas putida KU190
1988
Lee, Y.H. | Lee, Y.N. (Korea Univ., Seoul (Korea R.). Coll. of Science) | Joo, M.J. (Mokwon Methodist Univ., Taejon (Korea R.). Development of Microbiology)
The location of R-determinants, Ap/r and Tc/r, and replication origin in pKU41 was determined using the construction of miniplasmid by the BamHI and the HindIII restriction fragment from pKU41 and the cloning of the restriction fragments from pKU41 into pSY343. The gene encoding resistance to ampicillin (Ap) as well as replication origin in pKU41 were located on the region overlapping BamHI B fragment and HindIII A fragment. The gene encoding resistance to tetracycline (Tc) was located on the region of the HindIII C fragment, which was cleaved by BamHI as well
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