Purification of Citrus tristeza virus and production of antiserum and initial hybridomas for diagnosis of Citrus tristeza disease using ELISA [enzyme-linked immunosorbent assay] technique

Espino, T.M.; Exconde, S.B.; San Luis, M.M.; Ramirez, J.A. III . National Inst. of Biotechnology and Applied Microbiology

Purification of Citrus tristeza virus and production of antiserum and initial hybridomas for diagnosis of Citrus tristeza disease using ELISA [enzyme-linked immunosorbent assay] technique

1986

Espino, T.M. | Exconde, S.B. | San Luis, M.M. | Ramirez, J.A. III (Philippines Univ., Los Banos, College, Laguna (Philippines). National Inst. of Biotechnology and Applied Microbiology)

Citrus tristeza virus (CTV) was isolated and purified from diseased calamansi fruits. When examined under the electron microscope, the particles were shown to be long, flexous and threadlike, characteristic of CTV. The particle size ranged from 960 to 1780 x 10 nm. The average yield of CTV per 100 g sample was 727 microgram. Antiserum from CTV immunized mice showed positive results using enzyme-linked immunosorbent assay (ELISA) double antibody sandwich technique when tested with partially purified CTV from different varieties of CTV infected citrus plants. Initial hybridomas produced from fusion of immunized spleen and mycloma cells also showed positive results with ELISA technique. Further culturing of positive hybridomas is being done to propagate and mass produce them.

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