Laboratory diagnosis for sarcocystis asexual stages in cattle

Kang, Y.B.; Jang, H. . Veterinary Research Institute

Laboratory diagnosis for sarcocystis asexual stages in cattle

1988

Kang, Y.B. | Jang, H. (Rural Development Administration, Anyang (Korea R.). Veterinary Research Institute)

For the laboratory diagnosis of Sarcocystis infections especially in domesticated food animals, several antificial digestion techniques were applied for the musculature specimens and several staining techniques wes applied for the bradyzoites of Sarcocystis species isolated. The digestion technique using trypsin (0.5%) and sodium chloride (0.85%) mixed solution was regarded as the most valuable for the detection of asexual stages of Sarcocystis in bovine musculature specimens. Optimal time for digestion was approximately one to four hours. The trypsin digestion technique with Giemsa's stain could be helpful for the detection of Sarcocystis proliferative forms and for the observation of the nucleus of the parasite. A systematic detection was also performed in an autopsy for a bovine carcass naturally infected with Sarcocystis species, and the asexual stages such as metrocytes and bradyzoites were observed in the specific organs, respectively

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