Study on practical micropropagation of jujube cultivars through axillary bud culture
1988
Kim, D.S. | Lee, S.P. (Chungbuk Forest Resrarch Inst., Chongju (Korea R.))
This study was conducted to establish practical micropropagation of jujube cultivars ('Geumsung', 'Bokjo') by axillary bud culture. Addition of activated charcoal to half-strenght Murashige and Skoog(MS) medium supplemented with 0.5mg/l benzylaminopurine(BAP) enhanced shoot and root growth. At 500mg/l activated charcoal level 'Geumsung' showed best result, and shoot length and the number of multiple shoot were 6.4cm and 10.0, respectively. At 1,000mg/l activated charcoal level 'Bokjo' showed best result, and shoot length and the number of multiple shoot were 7.5cm and 12.4, respectively. As indole-3-butyric acid(IBA) concentration increased, rooting and callus growth of microshoot were enhanced. The optimum IBA concentration for shoot elongation and multiplication was 1.0mg/l. Growth responses of shoot-tip and axillary bud segments between two jujube cultivars were different. 'Geumsung' showed that axillary bud explants were about twice better than shoot-tip explants for shoot multiplication, but 'Bokjo' showed that shoot-tip explants were better than axillary bud explants for shoot elongation and multiplication. In acclimatization processes of plantlets produced in vitro, the survival of plantlets with only root primordia in soil medium was better than that of plantlets with several roots resulting in 97.8%. In cutting of in vitro-derived microshoot, paclobutrazol was more effective than IBA, naphthaleneacetic acid (NAA) and Rooton(R) in rooting and root growth
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