Ethanol production from starch by protoplast fusion between Aspergillus oryzae and Saccharomyces cerevisiae
1989
Lee, J.S. | Lee, S.Y. | Lee, Y.N. (Korea Univ., Seoul (Korea R.). Coll. of Science)
Amylolytic filamentous fungus, Aspergillus oryzae and nonamylolytic sugar fermentable yeast, Saccharomyces cerevisiae were fused by protoplast fusion in order to develope microorganisms having their integreated function. Amino acid auxotrophic properties were used as a genetic marker of protoplast fusion, and 35 % PEG 4000 was used as a fusogenic agent. Complementation frequengy of fusion was 4.6 x 10** (-6). Obtained fusants showed the morphology of yeast strains, the amylase activity and ethanol productivity. Among the properties of the fusants, morpholoy and prototrophic property were sustained stably but their ethanol productivity from starch was reduced. Although fusant strains had 0.50-fold ethanol productivity compared to that of S. cerevisiae in glucose medium, they produced ethanol from strach by direct fermentation
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