Construction of plasmids for overproduction of L-Phenylalanine
1990
Lee, S.B. | Park, C. | Won, C.H. | Choi, D.H. | Lim, B.S. (Miwon Cooperation Limited, Seoul (Korea Republic))
For the overproduction of L-phenylalanine using Escherichia coli, the authors constructed various recombinant plasmids including pMW 10, pMW 11 and pMW 12. The aroFFR and pheAFR genes for the production of L-phenylalanine were isolated from Escherichia coli MWEC 101-5 strains. The productivity and stability of Escherichia coli regulatory mutants containing recombinant plasmids were investigated to evaluate the efficiency of the aroFFR and pheAFR genes. The MWEC 101-5/pMW 11 strain produced 24.3 g/l of L-phenylalanine while its stability was 73.8 %. The specific activity of prephenate dehydratase in the MWEC 101-5/pMW 11 strain increased by 26-fold compared with that of Escherichia coli K-12
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