Evaluation of new rapid methods for aflatoxin detection in groundnuts in Zimbabwe
1989
Cole, D.L. | Masuka, A.J.
Two new rapid methods that use monoclonal antibodies to detect aflatoxins in groundnuts were tested. The Quantitox is an enzyme immunoassay using microtitre wells and the Aflatest is based on monoclonal antibodies adsorbed onto an affinity column. It was confirmed that aflatoxin contamination occurs preharvest and that storage under low-moisture conditions prevents any further toxin development. There were differences in susceptibility of the locally bred cultivars to aflatoxin development. The Aflatest procedure was useful to rapidly screen groundnut samples for aflatoxin contamination, but there were problems with the Quantitox method. Aflatoxin contamination occurred in the field prior to harvest and was not aggravated by storage conditions at Cleveland Depot during 1987. There was a 19.23 per cent incidence of aflatoxin contamination in the samples taken in early 1987, but it was not related to the farm management system (communal, small-scale commercial, and large-scale commercial) or any particular pest or disease
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