[Double diffusion, counter immunoelectrophoresis and indirect haemagglutination methods in the diagnosis of equine and swine fascioliasis]
1991
Gorman G, Texia | Bravo, J. (Chile Univ., Santiago (Chile). Fac. de Ciencias Veterinarias y Pecuarias) | Lorca, M. | Ibarra, L. | Llcaino C, Hector
The diagnostic efficiency of double diffusion (DD), counterelectrophoresis (CIE) and indirect haemagglutination (HAI) techniques in equine and porcine fascioliasis was evaluated using a crude adult Fasciola hepatica homogenate as antigen. Serum and faecal samples were obtained from 120 and 126 Fasciola hepatica infected horses and pigs, respectively, identified as such at post mortem abattoir inspection. The livers were examined and the number and size of flukes recovered were recorded. Faecal samples were analyzed by a standard quantitative coprological sedimentation method. Serum samples from non infected animals (43 horses and 50 pigs) were also obtained. The sensitivity of DD and CIE ranged from 7.5% to 12.7%, but their specificity reached 100% in horses an average of 97% in pigs. HAI detected 23.3% of horse infections and 84.9% of pig infections. Its specificity was only 83.7% in horses and 26% in pigs. Positive results were more frequent among horses with higher fluke burden (p0.05); this was not the case in pigs. Animals positive to DD and CIE tended to present smaller flukes than the negative (p0.05). We conclude that these methods when using a crude worm extract, are unsatisfactory for detecting chronic horse or swine fasciolasis. A significant correlation (p0.05) in horses (r
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