The micropropagation of carnation (Dianthus caryophyllus L.) to supply carnation seedlings
1986
Ernawati, A. | Gunawan, L. (Institut Pertanian Bogor (Indonesia). Fakultas Pertanian)
The possibility of using tissue culture techniques in preparing plantlets of carnation was studied, by conducting two experiments. The first one was designed to study the response of explant to plant growth regulators. Shoots from fields were used as explants. Basal medium consisted of Murashige-Skoog salts supplemented with BAP, KIN, IAA and NAA. Multiple shoots formed were observed in culture after 5 weeks and the highest number of which was observed in medium containing BAP 0.5 mg/l and IAA 0.1 mg/l. The second experiment was conducted to find the right combination of auxin for rooting and plantlets formation. Shoots from the first experiment were tested in media containing IAA, IBA, NAA and 2,4 D. The effect of the former plant growth regulators were also determined. Shoots obtained from various media in the first experiment were transfered to MS medium without growth regulators. Results indicated that roots formed in medium with 1 mg/l NAA, but the shoots were stunted. The highest number of healthy plantlets was induced in medium without growth regulator. Shoot inducing media also affected the percentage of healthy plantlets
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