Effect of desiccation and storage on the viability of Vicia faba pollen
1990
Telaye, A. | Beniwal, S.P.S. (Institute of Agricultural Research, Addis Ababa (Ethiopia)) | Gates, P. (University of Durham (United Kingdom). Department of Biological Science)
In a study on the storage of faba bean in three low temperature regimes and prior reduction of moisture by high temperature treatments, in vitro viability was studied in pollen of seven lines of Vicia faba after desiccating at 20 or 30 C and drying at room temperature (20.5 C) for 24h. A study on storage of fresh pollen and pollen predried at room temperature was also conducted over a period of one month. Storage was at -20 and -80 C or in liquid nitrogen (-196 C). Pollen grains desiccated at 30 C were least viable in desiccation experiments with significant variability among the lines. Line STW was affected least by such desiccation. In storage experiments, pollen grains, whether predried or fresh, were least viable when stored at -20 C and those stored in LN2 were the most viable. The results indicate that, with some significant variability among Vicia faba genotypes, desiccation at 30 C for even 6h has a detrimental effect on pollen viability. Pollen dried at room temperature for 24h is best stored in LN2. Reduction of pollen moisture prior to storage proved valuable in increasing pollen longevity in storage
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