Heterologous fertilization using livestock gametes
1992
Cox, J.F. (Cambridge Univ., Cambridge (United Kingdom). Animal Biotechnology Cambridge)
Using in vitro fertilization of in vitro-matured cattle, sheep and pig oocytes or in vivo-matured mouse oocytes, the present study was intended to evaluate the effect of the phylogenetic relationship between gametes on heterologous fertilization. In the Exp. 1, either denuded (-) or cumulus-enclosed (+) cattle and sheep oocytes were exposed to either ram (1 x 10 1.000.000 sperm/ml) or bull (0.6 x 10 1.000.000 sperm/ml) capacitated spermatozoa respectively under fertilizing conditions. In the Exp. 2, cumulus-enclosed cattle, sheep, pig and mouse oocytes were inseminated with bull capacitated spermatozoa (0.8 x 10 1.000.000 Sperm/ml) and allowed to interact under fertilizing conditions. The fertilization rate of the Exp. 1 based in the cytoplasmic presence of pronuclei and sperm tail, showed that the fertilization efficiency in heterologous systems involving ruminant gametes is high and that the cumulus exerted a simulatory effect on sperm penetration (p
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