Studies on the conidia formation of Pyrenophora teres Drechs, 1: Effects of medium, temperature and light quality
1990
Sato, K. (Okayama Univ., Kurashiki (Japan). Research Inst. for Bioresources) | Takeda, K.
Establishment of an artificial inoculation method is important for the investigation of the physiologic specialization and the host resistance of Pyrenophora teres, the causal organism of barley net blotch. Since P. teres is a poor sporulator in culture, it is essential for the artificial inoculation to establish a method of producing a large amount of conidia in culture. The present study was carried out to investigate the effects of medium, temperature and light quality for the growth and conidia formation of P. teres using isolates from Hokkaido and Canada. 1) The colonization was the greatest at 25 degrees C, although differences were observed among the 13 isolates. Colonization was different in the four media. 2) Sporulation was fair on V-8 medium, barley leaf medium and Misato-hara medium, but sparse on PDA medium and oatmeal medium, when four isolates were cultured at 25 degrees C under continuous black light blue (BLB) irradiation. 3) Cultured on Misato-hara medium under continuous BLB irradiation, two isolates from Hokkaido did not sporulate at 10 or 15 degrees C, while Canadian isolates sporulated at all five temperatures between 10 and 30 degrees C. Canadian isolates sporulated well when incubated at 25 degrees C interrupted by two day 10 degrees C treatment, whereas the low temperature treatment did not have a positive effect on isolates from Hokkaido. 4) cultured on Misato-hara medium at 25 degrees C, two isolates from Hokkaido did not sporulate under continuous fluorescent light or constant darkness, while Canadian isolates had sparse sporulation under the same condition. Long term irradiation by BLB had a positive effect on the sporulation of the four isolates examined
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