Thermal denaturation of squid [Todarodes pacificus] myofibrils: Effects of calcium ion and EDTA
1991
Konno, K. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries)
The effect of Ca2+ and EDTA on the thermal inactivation rate (KD) of squid mantle muscle myofibrils (Mf) ATPase was investigated, and squid Mf was found to be remarkably stabilized (roughly 100 times) in the presence of Ca2+ compared with in the presence of EDTA. The KD value of squid Mf was also affected by KCl concentration present, and increase in KCl from 0.1 M to 0.7 M enhanced the inactivation 500 fold. Therefore, it was concluded that squid myosin was stabilized significantly by actin in the presence of Ca2+ at a low-salt medium. It was also found that stabilizing effect of sorbitol was independent on the presence of Ca2+, and functioned additionally if both were present together
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