Leaf protein concentrates: Calopogonium caeruleum and Tithonia diversifolia
1988
Susana, I.W.R. | Tangendjaja, B. (Balai Penelitian Ternak, Bogor (Indonesia))
A study on the protein separation from other components (especially fiber) was conducted to produce Leaf Protein Concentrate (LPC) at the Research Institute for Animal Production. Several methods of protein separation have been investigated including coagulation of protein extract from Calopogonium caeruleum (kalopo) using heat treatment and acid precipitation of protein extract from Tithonia diversifolia (titonia). Recovery rate of LPC from the leaves on dry matter were 21 percent and 31 percent for kalopo and titonia respectively. The result on chemical analysis show that protein content increased from 18.9 percent in the leaf to 32.1 percent in LPC for kalopo and 21.6 percent in titonia leaf to 36.5 percent in the titonia LPC. Total Amino Acid (TAA) increased from 16.5 percent to 31.0 percent for kalopo and 18.8 percent to 35.2 percent for titonia. Tannin content increased from leaf meal and LPC of kalopo and titonia were 362, 247, 220 and 400 mg percent respectively. In vitro digestibility using pepsin-pancreatin showed that digestibility of LPC from kalopo and titonia were 96.8 percent and 89.7 percent respectively. This was significantly increased compared to the original leaves which were 56.5 percent and 49.0 percent respectively. Feeding trial with growing quails using Complete Randomized Design was performed in 5 replicates containing 5 birds/cage to evaluate the LPC from titonia. The dietary treatments were control without LPC ration containing 10 percent LPC, ration containing 20 percent LPC and ration containing 10 percent titonia leaf meal. Body weight gain in 4 weeks feeding did not give a significant difference among treatments and feed consumption is also not affected by treatments. This result indicated that LPC from titonia can be used in poultry ration up to 20 percent without adverse effect.
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