In vitro culture of derris (Derris elliptica (Wall.) Benth.)
1993
Rasco, S.M.
Tissue culture requirements of derris were investigated. For single-node and internode explants, decontamination was obtained through: pre-sterilization shoot incubation; use of explant sizes 5mm; flaming with 70% ethyl alcohol; double sterilization in series of solutions of chlorox and streptomycin sulfate. Forcing of decapitated shoots in the greenhouse was successful in distilled water. Axillary budbreaks were induced within the first week of incubation. The fast growing shoots obtained through forcing ensures ready supply of cleaner explants. WPM medium supplemented with 3.0 mg/l 2,4-D was identified for callus induction of shoot internodes. Shoot regeneration was not obtained for calli cultured in MS or WPM basal media supplemented with BAP, Ki and GA 3. MS of WPM with 3.0 mg/l BAP or 2iP and 10% coconut water was best for direct organogenesis of single-node explants. Rooting did not occur for all the regenerated plants. Tissue culture of derris is not viable, apparently within the limitations of this study, due to low explant decontamination, retarded shoot regeneration for alcohol propagation and unsuccessful shoot regeneration from calli
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