Inflorescence culture in rice
1993
Mandal, A.B. | Mohanraj, P. | Bandyopadhyay, A.K. (Central Agricultural Research Inst., Port Blair (India). Biotechnology Laboratory)
LS1, MS1, MS2, MS3, and MS4 failed to induce any calli, but direct plant regeneration without a visible callus stage was obtained in MS2, MS3, and MS4 with 2-cm-long young inflorescences. LS2, LS3, and LS4 induced callus formation. During peak callus formation, callus induction percentage and callus health varied significantly according to developmental stage of inflorescences. Calli formed earliest in young inflorescences (0.5 and 1.0 cm), which grew vigorously and had high induction frequencies of healthy calli. Induction percentage was inversely proportional to developmental stage of the inflorescence. LS3 was the best hormonal level for inducing calli. In the subculturing experiment, all calli died within 16-22 d, suggesting hormones might be an integral component of media for successful subculture. In the regeneration experiment, 2,4-D was completely withdrawn and replaced with IAA- appearance of green plantlets was fastest with 1-cm-long young inflorescences and slowest with 2-cm-long inflorescences. Regeneration frequency notably varied with developmental stage of the explant. Highest regeneration frequency occurred in 0.5 cm-long and lowest frequency in 5-cm long inflorescences. Young inflorescences are a good source for rice tissue culture. They have efficient callus induction and redifferentiation frequencies
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