Studies on the inhibition of Aspergillus parasiticus by Cladosporium fulvum in peanut, rice and corn
1993
Mabesa, R.C. | Lauzon, R.D. | Sumague, M.J.V. | Estioko, O.P. | Medrano, P.N. | Olivares, J.A.U. | Melendres, R.R. (Philippines Univ., Los Banos, College, Laguna (Philippines). Inst. of Food Science and Technology)
Cladosporium fulvum inhibited growth of Aspergillus parasiticus on artificial recovery media with peanut, rice and corn infusions. Inhibition was complete when C. fulvum was inoculated 7 days ahead of A. parasiticus in the infusions. Efforts to optimize mass production of C. fulvum in peanut, rice and corn dextrose broths through the shake flash culture technique were undertaken. Growth in these media was slow but rapid in malt extract broth. Consequently, C. fulvum was grown in this medium for mass production for toxicity evaluation. Toxicological studies using the chick embryo bioassay showed no harmful constitutents as indicated by 100% survival in the water soluble extract from medium, oil soluble extract from medium and oil soluble extract from C. fulvum. Determination of the mechanism of inhibition of C. fulvum was also conducted. Growth of Aspergillus parasiticus in potato dextrose agar was adversely affected by the culture filtrate and concentrated pigment from C. fulvum. Morphological changes include thinning and deformation of mycelium and reduction of spore count and size
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