Characterization of Two Isometric Grapevine Viruses of Austrian and Hungarian Origin
1993
Dimou, D.
A study was undertaken to compare two nepovirus isolates, F3 and H6 respectively of Austrian and Hungarian origin, that elicited yellow chrome mosaic symptoms in grapevine. The two isolates were compared with the type (H15) isolate of grapevine chrome mosaic virus (GCMV) of Hungarian origin. Biological and physico-chemical properties of the two isolates were determinated. Infected Chenopodium quinoa and Nicotiana occidentalis were the best sources for virus purification. The in vitro properties of the three isolates, were substantially similar with the exception of the slightly lower longevity of H6 isolate. The three isolates lost their infection capacity after dilution to 10-3/10-4 heating for 10 min at 60-62xC and storing for 10 days (7 for H6 isolate) at room temperature. In the electron microscope, the virus particles showed an angular profile and had a diameter of about 30 nm. No serological differences were observed between the three isolates in ELISA, ISEM and agar diffusion tests. No differences were observed in the number, type (ssRNA) and molecular weight of nucleic acids of F3 and H15 isolates, neither in the number and molecular weight (54 KDa) of coat protein subunits. F3 and H6 were recogniszed as GCMV isolate. Therefore, this represents the first report of the presence of GCMV in Austria. The F3 isolate did not differ from H15 isolate of GCMV. By contrast, isolate H6 showed differences in biological properties and behaviour during purification which distinguished it clearly from the other two GCMV isolates under study.
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