Meristem-tip culture of cocoa (Theobroma cacao L.)
1992
Adu-Ampomah, Y. | Novak, F.J. | Afza, R. | Van Duren, M. (International Atomic Energy Agency, Vienna (Austria). Agency's Laboratories, Plant Breeding Unit)
Meristem tips were excised from terminal and axillary buds of young cocoa plants (2-4 weeks old) and cultured on media with 1 mM zeatin and various concentrations of NAA and GA3. The subculturing of shoots in liquid medium was beneficial for nodal elongation and shoot growth. Gamma ray irradiation at low doses (1-4 Gy) helped to reduce callus formation. The roots were induced on half-strength MS medium with 10 mM IBA, 0.1 mM IAA and with reduced sucrose content (15000 mg/l).
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