In vitro effect of Clostridium perfringens enterotoxin on Vero cells and in vivo effects in animals
1993
Markovic, Lj. (Institute for Nuclear Sciences, Beograd - Vinca (Yugoslavia). Laboratory for Radiobiology and Molecular Genetics) | Asanin, R. | Dimitrijevic, B.
Clostridium perfringens type B was investigated for enterotoxin production. Enterotoxin was detected in the strain B L 361. Enterotoxin preparation was tested in vitro on Vero cells and in vivo on experimental animals. The enterotoxin induced erythema when injected intradermally into depilated guinea pigs and produced chronic alterations in the intestinal system of mice. The cumulative effects of Clostridium perfringens enterotoxin on Vero cells inhibited cellular metabolism and induced morphological changes in the cultured cells. These effects were more rapidly detected in the Vero cell system.
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