Comparison of the survival rate of in vivo and in vitro produced bovine embryos after a simple vitrification procedure
1994
Mahmoudzadeh, A.R. | Soom, A. van | Kruif, A. de (Gent Univ. (Belgium). Faculteit Diergeneeskunde)
Bovine in vivo and in vitro produced embryos at different stages of development were exposed to a vitrification procedure which has already resulted in a high in vitro survival rate for bovine in vivo compact morulae-early blastocysts. The vitrification solution consisted of 7.15 M ethylene glycol, 2.5 mM ficoll and 0.3 M sucrose (EFS solution). Compact morulae-early blastocysts, blastocysts and expanded blastocysts were transferred into straws and immersed into liquid nitrogen after 3 minutes exposure to EFS solution. The average post-thaw survival rate for the different stages of in vivo and embryos was 66.7 per cent, 15 per cent, 10 per cent, and 0 per cent, 4.8 per cent and 10.5 per cent, respectively. The overall survival rate of the in vivo and in vitro embryos was 38.6 per cent and 5 per cent, respectively. It is concluded that in vivo derived embryos are more resistant to vitrification and their average survival rate is significantly higher than of in vitro embryos (p0.05). The survival rate also depends on the stage of embryonic development. In vitro produced compact morulae-early blastocysts have a significantly higher survival rate than blastocysts and expanded blastocysts (p0.05). There exists also a significant difference between the average survival rate of compact morulae-early blastocysts and expanded blastocysts produced in vitro (p0.05).
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