Preliminary results of wheat polyhaploid production using wheat x maize crosses
1994
Giura, A. (Research Institute for Cereals and Industrial Crops, Fundulea (Romania))
Nine winter wheat F1 hybrids were used to produce wheat polyhaploids through intergeneric crosses with maize. Out of 6.230 pollinated florets, 75.0 percent formed seeds with a genotype variation of 64.3 to 86.8 percent. 66.9 percent of these were plump, well developed seeds ranged from 10.1 to 22.8 percent, indicating a genotype influence. The extracted embryos showed various shapes, sizes and degree of differentiation with great influence on the success of embryo culture. Out of 509 cultured embryos 233 germinated (45.8 percent) and 186 (36.5 percent) developed into plants. All cytological analysed plants had the polyhaploid chromosome complement of 2n = 21. After colchicine treatment 86 percent of plants survived and are being in the process of clones multiplication. With further improvement we hope that the intergeneric crosses wheat x maize may become a current procedure for a wheat polyhaploids production.
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