Evaluation of pedunculate oak (Quercus robur L.) populations in Slovenia
1994
Batic, F. | Sinkovic, T. | Javornik, B. (University of Ljubljana, Biotechnical Faculty, Ljubljana (Slovenia). Agronomy Dept.)
The article presents a taxonomic study carried out in three pedunculate oak populations in Slovenia by leaf morphometry and DNA analysis. About one hundred leaves were sampled from five trees from three seminatural pedunculate oak stands in the Subpannonian vegetation region of Slovenia. Samples were collected from the well lit upper part of the tree crowns, and only leaves from short twigs were analysed. Leaf samples were herbalised, and measured and assessed in the laboratory. Leaf dimension (lamina length and width, petiole length, number of lobe pairs) ware measured and characters like type of leaf pubescence and leaf lamina base type were assessed. RADP markers were used for genomic DNA analysis. Analysis of leaf morphometry showed that all sampled trees belong to the Quercus robur L. species. Nevertheless, on some trees, leaves also showed characters typical of sessile oak (Quercus petraea Matt. Leibl.), especially the leaf lamina base type and number of lobe pairs. Other parameters analysed were within the variation of pedunculate oak. The hybrid status of these trees was not confirmed by DNA analysis. More populations of both oak species should be analysed, especially when growing together, in order to obtain reliable data for taxonomic studies of oak hybrids and population genetic diversity by means of classic leaf and acorn morphometry and modern DNA analysis.
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