Germination, desiccation and cryopreservation of seeds of Populus deltoides Bartr.
1996
Pence, V.C. (University of Cincinnati, Cincinnati (USA). Department of Biological Sciences)
Seeds of Populus deltoides were examined for their ability to be dried under the air flow of a laminar flow hood and stored at room temperature, 4 deg C, -20 deg C and in liquid nitrogen. Seeds from dehiscent capsules germinated uniformly within one day, whereas germination of seeds from green (non-dehiscent) capsules was slower and took five to seven days to complete. Seeds of both types survived drying to below 10 % moisture, but lost germinability within two months if stored at ambient temperatures. Germinability was maintained over that time if storage temperatures were lowered to -20 deg C, but germinability dropped to between 40 and 70 % in green seeds stored at 4 deg C. These results suggest that storage at -20 deg C or in liquid nitrogen could be used for germplasm storage of Populus seeds
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