Evaluation of Kentucky bluegrass blends using isoelectric focusing and computer imaging
1995
Bell, G.E. | McDonald, M.B. | Danneberger, T.K. (The Ohio State Univ., Columbus (USA). Department of Horticulture and Crop Science)
Seeds of turfgrass cultivars are commonly blended to improve performance. Once physically blended, however, no fast, reliable technique is available to identify the proportions of cultivars present. The objective of this study was to test the effectiveness of computer imaging of electrophoretic gels for determining the relative proportions of two Kentucky bluegrass (Poa pratensis L.) cultivars in seed blends based on optical densities of electrophoretic bands unique to each cultivar. Blends of the two cultivars, "NuStar" and "Glade", ranging from 0 to 100 % were prepared by weight, protein extracted and separated on isoelectric focusing gels stained for esterase activity. The gels were electronically scanned and their electrophoretic images analysed using a computer software programme to determine the proportion of each cultivar present in 27 blends. The results indicated that this technique accurately quantified the percentages of cultivars in seed blends to within +/- 8.6 % with 95 % consistency. Based on this study, the combination of isoelectric focusing and computer imaging was an effective method for determining cultivar proportions in turfgrass seed blends
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