Some biological characteristics of rice somatic and zygotic embryos
1995
Xu Zhuyun | Wang Tongyu | He Meiying (Academia Sinica, Shanghai (China). Shanghai Inst. of Plant Physiology)
An analysis of protein extracts by SDS-polyacrylamide gel electrophoresis showed that the protein composition was similar in zygotic embryos (ZE) and the somatic embryos (SE) of rice cultured in vitro. About 17 bands were identical in the two embryo types according to their molecular weights. Among these bands, 67-94 kD polypeptides were present in much lower amounts in nonembryogenic calli (NE), and 39, 47 and 60 kD polypeptides were absent from NE. The amino acid components of free amino acid pool were also similar in ZE (7 d after anthesis) and SE. The predominant amino acids were Asp, Glu, Ala and Lys, and the Arg content increased in both embryo types during a later stage of differentiation, whereas the contents of these amino acids were lower in NE. In addition, it was found that the lectin-like substance occurred in both embryo types, but no hemagglutination activity was found in extracts from NE without embryoids
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