Effect of gamma radiation on in vitro cultured explants of yam (Dioscorea alata L.) cv. Kinampay
1993
Lapade, A.G. | Veluz, A.M.S. | Marbella, L.J. | Santos, I.S. (Philippine Nuclear Research Inst., Diliman, Quezon City, 1101 (Philippines). Atomic Research Div.)
Various explants of yam were irradiated with gamma rays at doses ranging from 5-50 Gy. Induction of callus was obtained in tuberous root and other vegetative explants: petiole, node, internode, and shoot apex. Callus induction was observed in the irradiated and unirradiated fleshy or tuberous root explants grown in Murashige and Skoog's (MS) medium with benzyl adenine (BA) in combination with naphthalene acetic acid (NAA) ranging from 2-6 ppm. Stimulation of callus growth was obtained from tuberous root explants irradiated with 5 and 10 Gy and grown in MS medium supplemented with 2 ppm BA in combination with 6 ppm NAA. Similar callus growth (as indicated by their weights) was observed at higher doses of 30 and 40 Gy, provided that higher levels of 4 ppm BA and 6 ppm NAA were incorporated into the MS medium. Similarly, induction, of callus was enhanced in tuberous root sections of putative mutant lines (for dwarfness and earliness) that were cultured in higher levels of BA and NAA in MS medium. Regeneration of plantlets was obtained from callus-derived shoot apex irradiated with 40 Gy and from calli-derived unirradiated nodal and tuberous root explants
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