Random amplified polymorphic DNA (RAPDs) from seeds of differing soybean and maize genotypes
1996
Jianhua, Z. (Shandong Academy of Agricultural Sciences, Jinan (China)) | McDonald, M.B. | Sweeney, P.M.
Random amplified polymorphic DNA (RAPD) banding patterns are useful in identifying genotypes of various crops. In particular, this technology has important applications in plant breeding, seed production, and seed testing programmes. However, before this potential is realized, studies must demonstrate that environmental factors do not influence RAPD results and that the test protocol can be standardized. This study showed that artificial and natural seed deterioration, contamination by fungi, and differing seed production environments did not generally affect the stability of RAPD markers in soybean (Glycine max [L.] Merr.) seed. RAPD markers obtained from maize (Zea mays L.) seed were dominant and hybrid seed generally possessed the same markers as both inbred parents. RAPD markers obtained from the maize seed coat and endosperm were not representative of their genetic heritage while those from the embryo were. The use of RAPD banding patterns appears applicable to a number of crops and can be successfully used to enhance and determine the genetic purity of individual seeds
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