Studies on isoenzyme patterns in some mutants of mungbean walet variety (Vigna radiata L. Wilczek)

anglais. Isoenzyme patterns of various enzyme active in metabolism of life organism, can be used as genetic alteration markers due to mutation. The aim of this study was to detect the differences in isoenzymes among induced mutants as well as between control and mutant of Walet variety mungbean and its four mutants (WI-1, WI-5, WI-6, and WII-15). Protein extracts of the leaves and raw seeds, were fractionated using polyacrylamide disc gel electrophoresis (concentration of gel 10 percent), then the electrogram were stained by the staining solution for esterase, peroxidase, and acid phosphatase isoenzymes. The zymogram showed that gene(s) controlling the production of enzymes have undergone mutation. The isoenzymes banding patterns of leaves were less sharp than those of raw seeds, and were difficult to be evaluated. While zymogram of different raw seed extract showed different peroxidase isoenzyme banding pattern, so that was reliable to be used as a marker to distinguish the differences between mutants and control, and also the differences among mutants of Walet variety mungbean

indonésien. Pola isoenzim dari berbagai enzim yang turut aktif dalam metabolisme suatu organisme hidup, dapat digunakan sebagai penyidik genetik termasuk terjadinya perubahan gen karena mutasi. Tujuan penelitian ini adalah untuk mendeteksi perbedaan antar-mutan dan mutan terhadap kontrol dari kacang hijau varietas Walet dan empat mutannya (WI-1, WI-5, WI-6, dan WII-15). Ekstrak isoenzim dari daun dan biji tanaman tersebut difraksinasi menggunakan elektroforesis disk gel poliakrilamid (konsentrasi gel 10 percent), kemudian elektrogram diwarna dengan pewarna isoenzim esterase, peroksidase, dan fosfatase asam. Zimogram menunjukkan bahwa gen pengontrol pembentukan enzim tersebut pada mutan yang telah mengalami mutasi. Pola pita isoenzim dari daun kurang tajam dibandingkan dengan biji mentah mutan kacang hijau menunjukkan pola pita yang beragam, sehingga selain untuk membedakan mutan-mutan terhadap kontrol, juga dapat membedakan antar-mutan yang berasal dari varietas yang sama