Biological control of root not fungus on Acacia mangium Willd.
1995
Mauricio, R.A.
The causal organisms was identified as Phellinus noxius based on the morphological characteristics. The fungus had brown and cottony mycelium and produced numerous setae. The antagonistic fungus used as T. viride. This produced hyaline and smooth walled spores. Growing the two organisms in-vitro showed no inhibition zone produced. T. viride parasitized P. noxius. The hyphae of P. viride coiled around and penetrated the hyphae of P. noxius. Both P. noxius and T. viride grew in potato dextrose agar, oatmeal agar, malt aextract agar and corn-meal agar. The former did not produce basidiomes in-vitro culture while the latter produced green tufts of conidiophores. They grew in a wide pH range of 5-9. Their growth was affected by different light temperature. Continuous darkness resulted to maximum mycelial growth in P, noxius in contrast to least mycelial growth of T. viridae. Rich nutrient (dextrose in media) produced better mycelial growth of both fungi. Conidial production of T. viridae was moderate to abundant. Mangium woodlocks (1" x 1" x 1", 1" x 1" x 4" and 1" x 1" x 6") were partially covered by brown mycelium after three months exposure to P. noxius. The weight losses of mangium woodblocks were significantly affected by the different treatments except for 1" x 1" x 4" woodlocks. The moisture content, compression parallel to grain and hardness of mangium woodlocks were not affected by the different treatments. The height, diameter and nodule count of P. noxius-inoculated mangium seedlings sprayed with either spore-mycelial suspension of T. viride or Captan were signficantly higher than those with P. noxius-inoculated mangium seedling. The shoot biomass was affected by the different treatments while the root biomass was not affected by the different treatments
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