Micropropagation of the hybrid papaya "Sinta"
1995
Mercado, J.C. | Villegas, V.N. | Pimentel, R.B. | Valencia, L.D. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
"Sinta" is a yellow hybrid papaya with very good fruit quality that is moderately tolerant to papaya ringspot virus (PRSV). Nutritional and growth regulator requirements for micropropagation of this papaya hybrid were established using varying levels of NAA, BA, GA3, sucrose and two basal media (MS and de Fossard). Parameters used in evaluating in vitro growth response were number and length of internodes, number of leaves, shoot and shoot weight. Optimum shoot growth was obtained using full-strength MS + 0.125 microM BA + 5 micro M GA3 + 58 micro sucrose. Full-strength de Fossard medium containing similar hormonal and sucrose levels gave a comparable response. Micropropagation opens the possibility of producing virus-free "Sinta" for transport to PRSV-free islands
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