Rapid detection of aflatoxin by serological methods
1989
Chintana Chana | Pranee Hamelink | Chiradej Chamswarng (Kasetsart Univ., Bangkok (Thailand). Faculty of Agriculture. Dept. of Plant Pathology)
Production of antiserum using antigens was done as follow: Aspergillus flavus (AFC); non aflatoxin producing isolated in Thailand; A. flavus (AFB 5), high aflatoxin producing isolated in Thailand; A. parasiticus (AP 827), high aflatoxin producing isolated from U.S.A.; Aflatoxin B1 (AF). After collecting normal serum, antigens were prepared and injected in 5 rabbits. Antisera were collected at weekly interval 10-20 time. The titers of antisera could not be detected by precipitation reaction on slides and in gels. The titer test will be repeated again by sensitive methods of immunoelectronmicroscopy (IEM). It is planned to detect aflatoxin by enzyme-linked immunosorbent assay (ELISA). Anti-aflatoxin B1 monoclonal antibodies AF1 and AF3 received from Prof. Yoshio UENO, Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Ichigaya, Tokyo 162, Japan, will be used in a pre-test experiment.
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