Tissue culture of date palm
1992
Phonthip Wongkaeo | Bunruan Phian-ngan | Thitiphon Phonthamphithak (Khon Kaen Univ., Khon Kaen (Thailand). Faculty of Agriculture)
2 years old date palm trees were chosen as source of explants for tissue culture studies. Explant tissues from leaf primodia, shoot apex, cotyledon and root of seedling and from young leaf, meristem tip, epicotyl, hypocotyl and root of 2 years old palm tree were used in the test for growth and development in selected culture media. Each tissue explant was subjected to MS medium containing various combination for 0.0-10.0 mg/l of NAA, BAP, 2iP, 2,4-D and kinetin. Most of cultured tissues have swollen to about 4 times of original cutting size in all media tested. However, the polyphenol exudate from tissue explants that gradually accumulated in culture medium has become toxic to the tissues themselves. So, the tissue explants should be transferred to fresh media every 2 weeks. The addition of activated chacoal to 0.3 % in the medium could prolong the survival of tissue explants in the culture for another 2-4 weeks. Callus initiation was best obtained from root tissues in MS medium containing NAA 0.5 mg/l and BAP 0.5 mg/l that were cultured in the dark at 29 deg C but development of embryogenic cells and shoot bud has not yet appeared. Plantlet production has been found in case of shoot apex cultured in MS medium supplemented with NAA 1.0 mg/l and kinetin 10.0 mg/l for shoot stimulation and NAA 0.1 mg/l for root differentiation, respectively
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