Evaluation of intradermal test for diagnosis of fascioliasis in buffaloes
1988
Swarup, D. (Indian veterinary Research Institute, Izatnagar (India). Div. of Experimental Medicine and Surgery) | Pachauri, S.P.
Based upon skin hypersensitivity reaction, intradermal test was applied for the diagnosis of spontaneous fascioliasis in buffaloes. A supernatant of homogenised and sonicated extract of F. gigantica was used as antigen. Selecting neck site region as the test site it was noticed that 48.78 %, 63.41 % and 70.74 % of infected buffaloes showed positive reactions at 1/2 h, 6 h and 24 h after intradermal inoculation of antigen. The histopathological examinations of skin biopsies taken from the test site revealed hypersensitivity-l and lll at 1/2 h and 24 h intervals, respectively. Maximum number of false reactions were noticed at 1/2 h period and in tuberculin positive herd and least at 24 h. The intradermal test produced better results when the readings were taken at 24 h and the values of =9.90 mm increase in skin fold thickness were considered as standard for positive reactions.
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