Effects of halomethyl-1, 3, 5-triazines on nitrification
1997
Ohki, S. (Tamagawa Univ., Machida, Tokyo (Japan)) | Kasahara, Y. | Murakami, M. | Miyamoto, Y. | Tokuyama, T. | Vonk, J.W. | Sato, Y. | Wakabayashi, K.
To evaluate the effect of the halogenated methyl groups of 1,3,5-triazines on nitrification by nitrifying bacteria, twenty-six halomethyl-1,3,5-triazines were examined. Nitrification inhibition activity by the 1,3,5-triazines was determined through two experiments; i.e. The inhibition activities wee measured for ammonia-oxidation to nitrate (N03(-)-N) in an upland soil and in the second one, for ammonia-oxidation to nitrite (NO2(-)-N) by Nitrosomonas europaea ATCC 25978. The 1,3,5-triazines bearing trichloromethyl group(s) inhibited nitrification more strongly both in soil and in cell suspension of ATCC 25978 than other partially chlorinated methyl-1,3,5-triazines. The 1,3,5-triazines having tribromomethyl group(s) exhibited rather weaker nitrification inhibition in soil, although they indicated the strong inhibition in cell suspension. The halomethyl-1,3,5-triazines in this study inhibited ammonia-oxidation step, but did not inhibit hydroxylamine-oxidation step in the culture of ATCC 25978. This fact was found that the inhibitory target site of them may be on the ammonia-oxidation step from ammonium (NH4(+)-N) to hydroxylamine (NH2OH), as we found for trichloromethyl-1,3,5-triazines in our previous study
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