Planting material production and in vitro conservation of garlic (Allium sativum L.)
1997
Patena, L.F. | dela Vina, A.C. | Bariring, A.L. | Tejano, M.P. | Barba, R.C. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
The AVNET Garlic Micropropagation System was used to produce planting materials for distribution to cooperators and for in vitro conservation. This system is now ready for trial (for Ca. 4-5 years) with a private company to develop a viable commercial technology. GO (generation O) or in vitro bulblets were better than in vitro plantlets as materials for outplanting since they survived better and were easier to handle and store. Trials showed that the best time to transplant these materials to soil was from August to November under highland (Baguio), and from November to December under lowland (Los Banos) conditions. Clove production (G1 to G2, 1996-1997 season) under field condition was 1:4-8 using tissue-cultured compared to 1:3-5 using non-tissue-cultured materials. At present, 9,694G2 or generation 2 bulbs grown GO bulblets were successfully produced. GO bulblets were also the materials chosen for conservation. They can be stored up to two months at 8-10 deg C. A study to conserve them longer than two months is on-going. Isozyme markers are being established to characterize each accession/variety and evaluate the genetic fidelity of these tissue-cultured materials. Preliminary results showed that in vitro culture did not alter the genetic composition of the accessions as exhibited by the absence of variation among tissue culture derived plants within accession. In addition, esterase band Ilocos white and Batanes revealed that these garlic accessions were genetically similar however, genetic differences were observed in shallot accessions
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