Detection of ratoon stunting disease in sugarcane
1998
Molina, G.C. | Dolores, L.M. | Tocino, P.L. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
Ratoon stunting disease (RSD) caused by the bacterium, Clavibacter xyli subsp xyli is a serious disease of sugarcane. Since one of the major transmission of this disease is through infected planting materials, it is essential to detect infection particularly on plants that serve as source of planting materials. Direct and indirect techniques to detect RSD were evaluated. The direct technique includes the observations of symptoms of the disease and microscopy. Stunting, poor growth and internal discoloration are among the characteristic symptoms of RSD infected sugarcane plants. Microscopic observation involves the examination of the xylem sap for presence of bacterial cells of C. xyli subsp xyli with the use of phase contrast microscope. The cells of the bacterium are small, thin and rod shape. Indirect techniques are the serological methods of detecting RSD. Two serological methods were evaluated, namely, the dot blot immunoassay and evaporative ELISA. Both techniques proved positive the identification of C. xyli subsp xyli infection on test plants as well as pure culture. Uninfected and resistant plants showed negative results. Results thus indicate that production of healthy planting materials through indexing using serological technique is possible
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