Genetic and molecular analysis of a cultivar specificity locus from the rice blast fungus Magnaporthe grisea
1996
Leong, S.A. | Farman, M.L. | Nitta, N.
Magnaporthe grisea cultivar specificity gene toward rice cultivar CO 39 was previously mapped to one arm of chromosome 1 between restriction fragment length polymorphism (RFLP) markers CH5-120H and 5-10-F. These RFLP markers map 11.6 and 17.2 cM, respectively, on either side of AVRCO39. Using Achilles' cleavage methods, CH5-120H and 5-10-F were shown to be separated by 600 kb. A chromosome walk to AVRCO39 was initiated from these flanking RFLP markers. More than 500 kb have been covered in 20 walking steps. The DNA conferring avirulence has been delimited to a 7.2-kb region. Preliminary hybridization studies on DNA of the virulent M. grisea strain Guy11, using portions of the 7.2-kb region, suggest that an alternate is not present, or if present, has undergone extensive mutation
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