Genetic modification of a rice glutelin cDNA and expression of the engineered glutelin gene in transgenic rice plants
1996
Moon, E. | Wu, R.
Rice provides up to 80% of the total calories and protein for the people of Asia. To improve the nutritional quality of rice, we modified a rice glutelin gene.Synthetic oligomers and a modified synthetic gene coding for essential amino acids were inserted into a glutelin cDNA. These engineered glutelin genes were introduced into a rice plant using a particle gun, and the transformants were regenerated. Production of the novel proteins in transgenic rice plants will be analyzed when they produce the seeds. For the maximum expression of the engineered gene in rice seeds, we are constructing a series of strong promoters. The strength of each promoter is being tested in immature rice seeds by in situ transient expression assay using the particle gun. We are also modifying the glutelin gene to provide the better target site for the insertion or fusion of useful DNA
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