Isolation and characterization of lipoxygenases from rice induced by incompatible infection of rice blast fungus, Magnaporthe grisea
1996
Peng, X.L. | Qin, Y. | Yang, Y. | Huang, J.
It has been previously reported that incompatible infection of rice blast fungus (Magnaporthe grisea) induces increased activity of lipoxygenases (LOXs) in rice. LOXs from incompatible and compatible M. grisea-infected rice leaves were compared by DEAE-Toyopearl column chromatography. It was found that leaf-LOX 3 was markedly induced in response to incompatible infection of the pathogen. This leaf-LOX 3 was further separated into two isoforms (CM-LOX 1 and CM-LOX 2) through CM-Toyopearl column chromatography. The two LOXs were then purified to homogeneity by gel filtration and Mono-Q chromatography. Sodium dodecylsulfate polyacrylamide gel electrophoresis showed a single polypeptide with a molecular weight of 98 kDa for CM-LOX 1 and 102 kDa for CM-LOX 2. Optimal pH was 5.5 for CM-LOX 1 and 6.5 for CM-LOX 2. The Km's of CM-LOX 1 and CM-LOX 2 were 115 and 54 mM for linoleic acid, and 73 and 57 mM for linoleic acid, respectively. The study also indicated that the two LOXs are immunologically related
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