Isolation and characterization of genes that control inflorescence
1996
Kushalappa, K. | Suresh Kumar, M.B. | Patwardhan, L. | Vijayraghavan, U.
Analysis of the development of rice inflorescence is being carried out using various molecular genetic techniques. Scanning electron microscopy (SEM) is being used to study various stages in panicle development. We report a SEM characterization of a wild-type flower and two mutant spikelets: multiple pistil 1 (mp1) and multiple pistil 2 (mp2). In both mutants, an increased number of stamen and carpel primordia was observed due to a primary defect in the size of the floral meristem. We have cloned from rice cDNAs fragments of two genes that contain the conserved MADS domain found in floral organ identity genes of other species. We have also cloned a fragment of a rice cDNA that bears homology to the conserved domain of the Arabidopsis gene, LEAFY, which is required for establishment of the floral meristem. The presence of a homolog for the LFY gene product in rice also has been demonstrated through immunohistochemistry. Antibodies raised against the Arabidopsis LEAFY protein detect the presence of a nuclear-localized homolog in sections of developing rice panicles
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