Comparison of RAPD linkage maps constructed for a single longleaf pine from both haploid and diploid mapping populations
1996
Kubisiak, T.L. | Nance, W.L. (Southern Inst. of Forest Genetics, Saucier (USA). USDA Forest Service, Southern Research Station) | Nelson, C.D. | Stine, M.
Considerable concern has been voiced regarding the reproducibility-transferability of RAPD markers across different genetic backgrounds in genetic mapping experiments. Therefore, separate gametic subsets (mapping populations) were used to construct individual random amplified polymorphic DNA (RAPD) linkage maps for a single longleaf pine (Pinus palustris Mill.). A haploid mapping population consisting of megagametophytic DNAs from 88 wind-pollinated seeds of longleaf pine (clone 3-356), and a diploid population including 86 F1 progeny from a controlled cross [longleaf pine 3-356( )x slash pine H-28( )]were employed. Seventy-one RAPD primers selected for this study identified a total of 137 mapped loci in longleaf pine 3-356 based on amplification of megagametophytic DNAs. Of the 137 loci useful for comparative purposes, 62 loci (45.3%) could not be scored when DNAs of F1 progeny from the controlled cross were amplified. Of the 75 loci that were scorable, 26 loci (34.7%) were fixed in slash pine H-28, and 49 loci (65.3%) were segregating (421:1, and seven 3:1). Comparisons were made using the 49 loci common to both the haploid- and diploid-based maps. The 49 loci allowed us to determine homologous counterparts between maps. Orders were conserved for those groups containing three or more loci. Genetic distance estimates were found to vary considerably, but not in any systematic manner
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