Effects of gamma radiation on variation in agronomic characters of aromatic rice (Oryza sativa L.) derived from tissue culture.
1993
Pudcharaporn Tangman
Tissue culture technique was used in conjunction with gamma irradiation for the induction of mutation in aromatic rice. Seeds of aromatic rice Khao Dawk Mali 105 were cultured on MS agar medium supplemented by cytokinin to induce multiple shoot formation. Results revealed that BA at the concentration of 25 mg/1 induced the highest percentage of multiple shoot - forming seeds and the largest number of shoots per seed, 93.3 % and 8.68 % shoots per seed, respectively. Shoots were rooted 100 % when transfered to the same medium without growth regulator or supplemented with IAA or IBA at the concentrations of 0.5 or 1.0 mg/1. Seeds were irradiated by different doses of gamma ray before cultured on MS medium supplemented with BA 25 mg/1 to induce multiple shoot formation. The increased dose of gamma ray from 0 to 240 Gray induced the higher percentage of multiple shoot - forming seeds from 42.5 to 75.0 percent and decreased at the higher doses. However the increased does of gamma ray from 0 to 400 Gray increased the number of shoots per seed from 5.7 to 21.1 shoots but inhibited at 440 Gray. Gamma ray at a dose of 380 Gray reduced the number of multiple shoot - forming seeds to 50 percent (RD 50). The multiple shoots were formed roots and the complete plantlets (M1 R1) were obtained. Results of variation in M1 R1 plants revealed that the number of tillers and panicles per plant were increased while the dose increased. But the plant height, number of fertile panicle and percentage of seed fertility were decreased when the dose increased.
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