Virus diseases of garlic and their control
1993
Suwanna Kladpan | Rujiporn Prateepasen | Yupa Mongkolsook (Kasetsart Univ., Bangkok (Thailand). Kasetsart Univ. Research and Development Inst. Central Scientific Eqnipment and Laboratory Center)
Diseased garlics were collected from Chiang Mai and Si Sa Ket provinces. After detection and identification of virus particles by IEM and ELISA techniques, two types of flexous rods particles were found; a longer one with 700-800 nm in length and a shorter one with 600-650 nm in length. The longer showed serological related to antiserum of Leek yellow stripe virus, the shorter to antiserum of Shallot latent virus both viruses showed serological unrelated to Onion yellow dwarf virus. The viruses can be found both in diseased and symptomless plants, and usually occur in mixed infection. From electron microscope, cylindrical inclusions; pinwheel and scroll, were also detected in cytoplasm of infected cells. Attemps of virus isolation and experimental host range study were tried but both viruses showed local lesion symptom only on Chenopodium amaranticolor. After treating infected garlic plants under heat at 38+-2 deg C for 1 month, percentage of regenerated plants was 35 and virus-free plants was 20. By submerging infected garlic heads under water overnight at room temperature, followed by 50 deg C water for 120 minutes, percentage of regenerated plants was 36 and virus-free plants was up to 90.
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