Cloning and mRNA transcription analysis of five developmentally regulated cDNA families in sunflower immature embryos
1997
Kabbaj, A. | Berville, A. (INRA-Montpellier, Montpellier Cedex 1 (France). UFR Genetique et Amelioration des Plantes)
In order to isolate and to analyse the expression of developmentally regulated genes in sunflower, cDNA libraries were constructed using mRNA from 12 or 20 day-old seeds and then differentially hybridized with mRNA from dry seeds and leaves. cDNA encoding major storage proteins (helianthinin and albumin Met-rich), proteinase inhibitor and two other cDNA with unknown function were isolated. The steady state levels of the transcripts, detected with each cDNA, were determined in immature seeds and vegetative tissues at different stages. The accumulation of the five mRNAs was tissue specific, limited to the mid-maturation stage of seed development. The albumin Met-rich mRNA accumulation occurred relatively later than the accumulation of both the helianthinin mRNA and the previously studied HaG5 albumin mRNA in sunflower. The accumulation of helianthinin transcript was higher in the CANP3 normal variety in comparison with the HOC "high oil and oleic" line. The levels of the albumin Met-rich mRNA were in contrast similar in both lines
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