Binding patterns of IgE antiibodies in sera of rubber tappers to fresh hevea latex serum proteins
1998
Hasma, H. (Rubber research Institute of Malaysia, P.O.Box 10150, 50908 Kuala Lumpur (Malaysia)) | Shahnaz, M. | Yip, E. | Azizah, M. | Mok, K.L. | Nasuruddin, B.A.
The binding patterns of IgE antibodies to fresh natural rubber latex-B and C-serum proteins were determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis immunoblotting technique. All the IgE samples were from Malaysian rubber tappers who had been skin-prick tested with extracts of gloves and food: avocade, potato, tomato and watermelon. Two of the five IgE samples from tappers reacting to gloves bound to latex proteins, specifically to 35, 38 and 40 kD B-serum proteins and to 30 kD and 75 kD C-serum proteins. The remaining three either did not bind or bound faintly to the latex proteins. Similar binding pattern to only a few latex proteins was shown in one of the three sera of tappers reacting to both gloves and food. The other two, however, exhibited multiple bindings to a wide variety of B-serum proteins of molecular weights less than 20 kD to greater than 202 kD and to a number of C-serum proteins between 30 kD to 75 kD. The heterogenus binding pattern was also demonstrated by eleven of the twenty IgE serum samples of tappers reacting to food and by twenty-five of the hundred and thirty-six serum samples of tappers reacting negatively to both gloves and food. The fact that only two of the thirty-eight serum samples that showed strong multiple binding pattern corresponded to a positive skin-prick test to gloves, indicated that in vitro immunoblotting using IgE antibodies in human blood is an unreliable of latex allergy.
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