Screening of potato germplasm for resistance against major potato viruses under field conditions
1995
Ahmad, M. | Ahmad, W. (Ayub Agricultural Research Inst., Faisalabad (Pakistan). Plant Virology Section)
One hundred and forty eight potato clones/germplasms were screened at Murree and Faisalabad for the detection of potato leaf roll virus (PLRV), potato virus Y (PVY) and potato virus X (PVX) by using enzyme linked immunosorbent assay (ELISA). The potato leaf samples collected from Murree were found infected with PLRV at the rate of 3.7% in the material produced through local crosses, 28.56% in the imported material other than International Potato Centre (CIP) material. CIP material and commercial varieties were found free from PLRV. PVY was present at the rate of 28.56% in CIP material, 14.28% in the imported material other than CIP. Material produced through local crosses and commercial varieties were found free from PVY. PVX infection was detected in CIP material (64.26%), imported material other than CIP (14.28%), local crosses (18.50%) but all the commercial varieties were found free from this virus. Similar studies were conducted at Faisalabad during autumn season 1994. CIP material, local crosses and commercial varieties were found free from PLRV. However, PLRV was detected @ 3.84% from imported material other than CIP. PVY was detected in CIP material (29.87%), local crosses (40.00%), commercial varieties (40.00%) and imported material other than CIP (15.38%). PVX was detected in 18.18% material from CIP, 50.00% in local crosses, 220.00% in commercial varieties and 11.53% in imported material other than CIP.
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