In vitro culture of dahlia (Dahlia hybrid)
1996
Kularb Kongthong
Shoot tips and lateral buds of 5 varieties of dahlia, Red Skin, Unwins Dwarf Hybrid, Double Cactus Mixed, Dapper Mixed and Sunny Yellow were established on Murashige and Skoog (MS) medium supplemented with BA and NAA at the concentration 0-0.5 mg/l. The highest number of shoots were induced at 0.5 mg/l BA for Red Skin, 0.1 mg/l BA + 0.1 mg/l NAA for Unwins Dwarf Hybrid, 0.3 mg/l BA for Double Cactus Mixed, 0.1 mg/l for Dapper Mixed and at 0.5 mg/l for Sunny Yellow. At 0.5 mg/l BA + 0.1 mg/l NAA callus at the base of stems of Double Cactus Mixed was initiated and can regenerated on 0.1 mg/l BA and 0.5 mg/l BA + 0.1 mg/l NAA. Shoot tips and nodes were cultured on MS medium supplemented with various concentration rate of NAA. The highest percentage of rooting and the best roots were produced at 0.15 mg/l NAA. The survival percentage of dahlia plantlets were 60 percent when transplanted. Lateral buds, leaves and young flowers of Dwarf Double Mixed were cultured on MS medium supplemented with BA and NAA at the concentration 0-0.5 mg/l. Lateral buds produced new shoots for all treatments. Callus was initiated by culturing young leaves on BA and/or NAA and rooting from leaves obtained by culturing on 0, 0.1, 0.3 and 0.5 mg/l NAA. Young flower buds can developed on 0.5 mg/l BA + 0.5 mg/l NAA. Young leaves were cultured on MS medium supplemented with BA and 2,4-D at the concentration 0-0.2 mg/l. The most effective for produced callus, obtained by culturing young leaves on 0.1 mg/l BA + 0.05 mg/l 2,4-D, but callus did not regenerate.
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